Journal: Frontiers in Microbiology

Article Title: Designing Synergistic Biostimulants Formulation Containing Autochthonous Phosphate-Solubilizing Bacteria for Sustainable Wheat Production

doi: 10.3389/fmicb.2022.889073

Figure Lengend Snippet: Re-isolation and detection of inoculated P-solubilizing bacteria. The CLSM of wheat roots at 35 days post inoculation of P-solubilizing bacteria in the pot experiment under net house conditions. The FLUOS-labeled oligonucleotide probes showed green fluorescent signals for total bacterial population and Cy3-labeled oligonucleotide probes showed red fluorescent signals for inoculated Ochrobactrum sp. SSR. (A) Control treatments without inoculation and from the PSB inoculated pots with carrier materials (B) Biochar, (C) CM, (D) FM, and (E) HA after hybridizing with fluorescently labeled probes. The plant growth promoting traits of re-isolated PSB strains for their confirmation in comparison to pure PSB (F) . The plates depicting the re-isolated colonies of SSR, ZW32, and ZW9 from BC (G) , CM (H) , FM (I) and HA (J) in inoculated treatments. Enterobacter spp. ZW9, ZW32, Ochrobactrum sp. SSR. B, bacteria; RC, root cells.

Article Snippet: These PSB are non-pathogenic, compatible with each other and were also submitted to DSMZ ( https://www.dsmz.de/ ) culture collection under the accession numbers DSM 109610 and DSM 109592 , DSM 109593 for Ochrobactrum sp. SSR, Enterobacter sp. ZW9 and Enterobacter sp. ZW32, respectively.

Techniques: Isolation, Labeling

Journal: The Journal of Experimental Medicine

Article Title: Suppression of local type I interferon by gut microbiota–derived butyrate impairs antitumor effects of ionizing radiation

doi: 10.1084/jem.20201915

Figure Lengend Snippet: Vancomycin decreased Lachnospiraceae in the gastrointestinal tract, while both Lachnospiraceae supplementation and butyrate administration impaired the antitumor effect of IR, in a melanoma model. (A) Verification of the abundance of Lachnospiraceae by qPCR using Lachnospiraceae (TSD-26) genomic DNA as a standard ( n = 4/group). Unpaired t tests were used to analyze the data. (B) GF C57BL/6 mice ( n = 5/group) were fed Lachnospiraceae and K. alysoides (TSD-26) for 1 mo, 10 8 CFU/mouse weekly. The control group was fed the same volume of autoclaved water. Then the mice were injected s.c. with 10 6 B16F1 cells, and established tumors were irradiated (20 Gy) on day 10 after tumor inoculation. (C) C57BL/6 mice ( n = 5/group) were injected s.c. with 10 6 B16F1 cells. Established tumors were irradiated (20 Gy) on day 10 after tumor inoculation. Tumor-bearing mice were injected i.v. with 4 μmol of NaBu on days 1, 4, and 7 after IR. (D) C57BL/6 mice ( n = 5/group) were injected s.c. with 10 6 B16F1 cells. Established tumors were irradiated (20 Gy) on day 10 after tumor inoculation. Tumor-bearing mice were intratumorally (i.t.) injected with 2 μmol of NaBu on days 1, 4, and 7 after IR. A tumor growth curve is shown. Two-way ANOVA tests were used to analyze the tumor growth data. *, P < 0.05; ****, P < 0.0001. One representative experiment (out of two experiments) is shown.

Article Snippet: After gavage, K. alysoides (TSD-26; American Type Culture Collection [ATCC])–colonized mice had a decreased antitumor response to IR compared with control mice in both MC38 and B16F1 tumor models ( ).

Techniques: Control, Injection, Irradiation

Journal: The Journal of Experimental Medicine

Article Title: Suppression of local type I interferon by gut microbiota–derived butyrate impairs antitumor effects of ionizing radiation

doi: 10.1084/jem.20201915

Figure Lengend Snippet: Lachnospiraceae and butyrate restrict the antitumor effect of IR. (A) GF C57BL/6 mice were fed with Lachnospiraceae and K. alysoides (TSD-26) for 1 mo, 10 8 CFU/mouse weekly. The control group was fed the same volume of autoclaved water. Mice were injected s.c. with 10 6 MC38 cells, and established tumors were irradiated (20 Gy) on day 10 after tumor inoculation. On day 10 after tumor inoculation, sera were collected for butyrate detection by GC/MS. A tumor growth curve is shown ( n = 5/group). (B) GF C57BL/6 mice were fed HI TSD-26 weekly for 1 mo. 10 8 CFU of TSD-26 were HI at 100°C for 30 min and fed to one mouse. The control group was fed the same volume of autoclaved water. Then the mice were injected s.c. with 10 6 MC38 cells, and established tumors were irradiated (20 Gy) on day 10 after tumor inoculation. A tumor growth curve is shown ( n = 5/group). On day 10 after tumor inoculation, sera were collected for butyrate detection by GC/MS. (C) Butyrate concentration in sera collected from A and B (control group, n = 10; TSD-26 group, n = 10; HI TSD-26 group, n = 12). Data were pooled from two independent experiments. (D) C57BL/6 mice were injected s.c. with 10 6 MC38 cells. Established tumors were irradiated (20 Gy) on day 10 after tumor inoculation. Tumor-bearing mice were injected i.v. with 4 μmol of NaBu on days 1, 4, and 7 after IR. A tumor growth curve is shown ( n = 5/group). Two-way ANOVA tests were used to analyze the tumor growth data, and unpaired t tests were used to analyze the other data. n.s., not significant; *, P < 0.05; **, P < 0.01; ****, P < 0.0001. One representative experiment (out of two experiments in A and B or three experiments in D) is shown.

Article Snippet: After gavage, K. alysoides (TSD-26; American Type Culture Collection [ATCC])–colonized mice had a decreased antitumor response to IR compared with control mice in both MC38 and B16F1 tumor models ( ).

Techniques: Control, Injection, Irradiation, Gas Chromatography-Mass Spectrometry, Concentration Assay

Journal: The Journal of Experimental Medicine

Article Title: Suppression of local type I interferon by gut microbiota–derived butyrate impairs antitumor effects of ionizing radiation

doi: 10.1084/jem.20201915

Figure Lengend Snippet: HI Lachnospiraceae species do not affect intratumoral butyrate concentration. GF C57BL/6 mice ( n = 5/group) were fed HI Lachnospiraceae and K. alysoides (TSD-26) weekly for 1 mo. 10 8 CFU of TSD-26 were HI at 100°C for 30 min and fed to one mouse. The control group was fed the same volume of autoclaved water. Then the mice were injected s.c. with 10 6 MC38 cells. On day 10 after tumor inoculation, tumors (control group, n = 14; HI-TSD-26 group, n = 10) were collected for butyrate detection by GC/MS. Unpaired t tests were used to analyze the data. n.s., not significant. Data were pooled based on two experiments yielding similar results.

Article Snippet: After gavage, K. alysoides (TSD-26; American Type Culture Collection [ATCC])–colonized mice had a decreased antitumor response to IR compared with control mice in both MC38 and B16F1 tumor models ( ).

Techniques: Concentration Assay, Control, Injection, Gas Chromatography-Mass Spectrometry

Journal: Scientific Reports

Article Title: Antimicrobial potential of a ponericin-like peptide isolated from Bombyx mori L. hemolymph in response to Pseudomonas aeruginosa infection

doi: 10.1038/s41598-022-19450-8

Figure Lengend Snippet: Effect of different concentration of Bm -ponericin-L1 on ESKAPE pathogens. ( a ) E. faecium (ATCC 35667), ( b ) S. aureus (ATCC 6538), ( c ) K. pneumoniae (ATCC 70063), ( d ) A. baumannii (ATCC 17978), ( e ) P. aeruginosa (ATCC 10145), and ( f ) E. agglomerans (ATCC 27985). ( g ) SEM micrograph of intact/live bacteria (green arrows) and lysed/dead bacteria (red arrows) in the presence of Bm -ponericin-L1. Negative control (NC) and positive control (PC). The data were expressed as mean ± standard deviation and the significance level was set at p < 0.05.

Article Snippet: Enterococcus faecium (ATCC 35667) , Staphylococcus aureus (ATCC 6538) , Klebsiella pneumoniae (ATCC 70063) , Acinetobacter baumannii (ATCC 17978), P. aeruginosa (ATCC 10145), and Enterobacter agglomerans (ATCC 27985) belong to the ESKAPE bacterial group and were cultured in Mueller Hinton Broth (MHB) to determine the minimum inhibitory concentration (MIC) of the Bm -ponericin-L1 peptide using broth microdilution technique as per Clinical and Laboratory Standards Institute (CLSI) guidelines .

Techniques: Concentration Assay, Bacteria, Negative Control, Positive Control, Standard Deviation

Journal: Letters in Applied Microbiology

Article Title: Cadmium‐tolerant bacteria: current trends and applications in agriculture

doi: 10.1111/lam.13594

Figure Lengend Snippet: Some examples of culturable and viable cadmium‐tolerant bacteria/yeast/algae showed by the tolerance mechanism and the associated crop/niche system where they were isolated

Article Snippet: , Enterobacter sp. , Industry wastewater treatment plant , Lu et al . ( ) .

Techniques: Bacteria, Algae, Residue, Bioleaching